Lizard, snake T-cell mystery

The intriguing Australian sleepy lizard has raised new questions about vertebrate immunity after the surprise discovery of the evolutionary disappearance of genes needed for some T cell production in squamates.

A US-led international research group, including South Australian Museum, Flinders University and other experts, conclude it’s likely that all squamates (snakes and lizards) are missing one set of T lymphocyte cells – paving the way for new research into what these T cells do in other animals, and how squamates compensate for this difference in immunology.

The ‘Skinks Make Do without γδ T Cells’ cover article in the Journal of Immunology compares the sleepy lizard genome to other reptiles and found it likely all squamates have lost one of the two major types of T cells over time.

Professor Mike Gardner and Dr Jess Clayton with some of the sleepy lizards studied at Flinders University.

“Out of all animals, it’s rather odd snakes and lizards have survived without this complete set of disease-fighting cells, so this discovery could encourage further research on what these missing T cells actually do in vertebrates,” says co-author Flinders University Professor Mike Gardner, an expert in the squamate skink Tiliqua rugosa (commonly known as sleepy, bobtail or shingleback lizard).

SA Museum senior researcher Dr Terry Bertozzi, another author in the article, says the disappearance of certain T cells in squamates over time points also towards previously unexplored roles of other immune cells to compensate and protect against disease, inflammation and cancer.

The NZ tuatara, Sphenodon punctatus, which was part of the reptile genomics study.

Senior lead author, University of New Mexico Professor of Biology Robert Miller, says the natural deletion or knockout of this major but “enigmatic” T cell lineage raises many questions, in particular the skink’s reliance on just one T cell type.

“The genes encoding the αβ TCR chains in the skink do not appear to have increased in complexity to compensate for the loss of γδ T cells,” says Professor Miller, director of the Center for Evolutionary and Theoretical Immunology, University of New Mexico.

“Understanding how squamates have compensated for the loss of γδ T cells could inform us of yet undiscovered roles they play in the immune systems of species that have these cells, such as humans.

“Work with genetically modified mice that lack γδ T cells have revealed that other cells, such as αβ T cells attempt to compensate but not effectively. Perhaps other mechanisms have stepped in to compensate in the squamates.”

T lymphocytes or T cells are key components of the vertebrate response to pathogens and cancer. There are two T cell classes based on their TCRs, αβ T cells and γδ T cells, and each plays a critical role in immune responses.

Sleepy lizards have been studied for more than 30 years by Flinders University scientists. Photo: Mike Gardner, Flinders University.

Snakes and lizards may be unique among the vertebrate lineages by lacking an entire class of T cells, the γδ T cells.

The article, ‘Comparison of Reptilian Genomes Reveals Deletions Associated with the Natural Loss of γδ T Cells in Squamates’ 2022 by Kimberly A Morrissey, Jordan M Sampson, Megan Rivera, Lijing Bu, Victoria L Hansen, Neil J Gemmell, Michael G Gardner, Terry Bertozzi and Robert D Miller has been published in The Journal of Immunology DOI: 10.4049/jimmunol.2290004

Acknowledgements: This work was supported in part by US National Science Foundation and by Australian Research Council Discovery Grant DP200102880 (to M.G.G., R.D.M., and T.B.). Genome sequencing financial support came from New Zealand Genomics Limited, the Allan Wilson Centre, and the University of Otago and by BioPlatforms Australia under the Amphibian and Reptile Genome Initiative and Holsworth Wildlife Research Endowment.

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